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Patient eligibility Patients recruited into this study were required to have histologically confirmed non-small cell lung cancer. All patients were to have measurable disease target lesions on physical examination photographs ; , or computed tomography CT ; . Other eligibility criteria were age 70 years, World Health Organization WHO ; performance status PS ; 2 with a life expectancy 3 months, adequate bone marrow reserve absolute granulocyte count, 1, 500 L; platelet count, 100, 000 L; hemoglobin level, 10 g dL ; , as well as adequate renal and hepatic function serum creatinine, AST, ALT and bilirubin levels 1.25 times the upper limit of normal ; . Exclusion criteria were the presence of other concomitant or metachronous cancers, brain or leptomeningeal metastases, radiation to the only measurable disease or within 4 weeks of starting chemotherapy, a pre. Karin F Giordano, MD, is a fellow in hematology oncology at the Mayo Clinic College of Medicine in Rochester, Minnesota. Her areas of interest and research include lung cancer and end-of-life care. She received her medical degree from Dartmouth Medical School and completed her residency in internal medicine at the Mayo Clinic. Aminah Jatoi, MD, is Associate Professor in the Division of Medical Oncology, Department of Oncology at the Mayo Clinic College of Medicine in Rochester, Minnesota. As a practicing medical oncologist at the Mayo Clinic, she is actively involved in supportive care issues in patients with solid tumor malignancies. Dr Jatoi holds a longstanding interest in the cancerrelated anorexia and weight loss syndrome, as well as in other supportive care issues relevant to cancer patients. She is currently the principal investigator on several actively accruing clinical trials, for example, imovane weight!

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Id. See also Julian Guthrie, Sabin Russell, and Katherine Seligman, "After Daughter's Death, Father Wants Close Look at RU-486; Abortion Pill's Safety Defended by Doctors as Better than Surgery, " San Francisco Chronicle Sept. 20, 2003 ; : at A17 available at: : sfgate cgi-bin article ?file chronicle archive 2003 09 20 BA310011.DTL ; "Patterson said the attending physician at Pleasanton's Valley Care Medical Center told him his daughter had died of septic shock a severe bacterial infection. `The doctor told me she had fragments of the fetus still left in her uterus and that caused the infection.'.

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Results and Discussion The reaction scheme for the synthesis of prodrugs 2 and 3 and their activation to the parent compound 1 is shown in Scheme. 1. One-step reactions resulted in these compounds in high yield 80% ; and both compounds were easily obtained in high purity 99% ; by conventional HPLC methods. Both prodrugs show, surprisingly, exceptional solution stabilities for several weeks in formulations surfactantethanol, 1% saline combinations ; and under conditions encountered in clinical applications intravenous delivery ; . Under acidic conditions 1% HCl ; compound 2 converts to 3 within 2 h with some degradation about 10% ; of the glycoside moiety. In preliminary experiments at neutral pH in PBS buffered solutions, the prodrugs were very stable, but in the presence of plasma or serum both 2 and 3 hydrolyzed to 1, with the elimination of soketal and glycerine, respectively. When serum was fractionated by size exclusion chromatography G-200 column ; it was found that all the conversion to the parent drug occurred in a fraction of molecular weights between 80 and 40 kD, which was within the molecular weight range of carboxyl esterases. The kinetics of prodrug hydrolysis were then determined at 37 C PBS buffer at various pH, in human serum and in buffer solutions containing porcine liver esterase and nexium.

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Hyperbaric oxygen HBO ; therapy is successfully used to treat chronic wounds by stimulating tissue repair, including angiogenesis. Angiogenesis usually involves the stimulation of VEGF production in blood vessels, possibly via NO signalling, but the role of HBO in this process is controversial. This study aimed to investigate the effect of HBO on VEGF and NO production by blood vessels in vitro. Male Sprague Dawley rats 350 - 400 g; n 17 ; were killed by Sagatal 90 mg -1; I.P. ; . Sections of aorta were placed in culture dishes containing 6 ml of modified Krebs-Ringer solution in mM: NaCl 118.6, KCl 4.7, CaCl2 2.5, MgSO4 1.2, KH2PO4 1.2, NaHCO3 25.1, Hepes 10, glucose 10, pH 7.4 ; for 30 min before 90 min treatments. The treatments were air at 1 ATA control ; , 100% O2 at 1 ATA or 100% O2 at 2.2 ATA. After treatment the tissue remained in KrebsRinger for a further 4 hr in air. All experiments were conducted at 37 C. Tissue and medium samples were collected pre-treatment pre ; , immediately post treatment post ; , and 4 hr after treatment 4h ; . We measured LDH activity, [lactate], [NO2-] and [H2O2] in the medium; [VEGF] in the medium and the tissue by ELISA R&D Systems, MMV00 ; . All data are means S.E.M n 7-8 ; and analyzed using ANOVA. LDH release and lactate content increased over time, and were lower in the HBO group during and following treatment when compared with either the control or oxygen only groups. At 4h, LDH in the HBO group 11.4 2.7 U.g-1 protein ; was significantly lower P 0.05 ; than that in the control group 23.8 10.5 ; . Lactate in the HBO group at post and 4h 0.16 0.02 and 0.44 0.6 -1 mol.mg protein ; were significantly lower P 0.05 ; than the control 0.26 0.03 and 0.72 0.15 ; . H2O2 remained at nanomolar level and showed a transient rise peaking at post, and then declining not statistically significant between treatments ; . NO2- levels were stable throughout the experiment in all groups range from 1.67 to 2.13 nmol.mg-1 protein ; . [VEGF] in the medium remained at or below the detection limit 3 pg.ml-1 ; . VEGF in crude homogenates of blood vessels was easily detected and progressively increased reaching peak values by 4h 34.2 4.4, pg.mg-1 protein in controls, oxygen only and HBO groups vs. 15.7 5.5 pre level; P 0.05 ; , no treatment differences were shown. Overall we conclude that HBO does not damage the blood vessels as evidenced by low LDH leak and only nanomolar levels of H2O2 generation. No evidence for NO-induced VEGF release by a single HBO is shown from this study.
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