On studies using the single-pass intestinal perfusion technique in rats, results show that the uptake of VACV is potentially mediated by several transporters, including the intestinal peptide transporter Sinko and Balimane, 1998 ; . Studies using rabbit BBMVs, Caco-2 cells, or hPEPT1 Caco-2 cells also demonstrate that the uptake or permeability of VACV is concentration dependent and saturable Lee et al., 1996; Cook et al., 1997; Hu and Sinko, 1997; Han et al., 1998 ; . These studies were performed in experimental systems where multiple transporters are potentially present. In other words, intact tissues or Caco-2 cells possess multiple transporters that may potentially be involved in the uptake of VACV. Therefore, it is difficult to characterize and evaluate the role of a single transporter such as PEPT1 in the intestinal transport of VACV. Recently, uptake of VACV was also studied in hPEPT1 XLOs, hPEPT1 HeLa cells, or rPEPT2 HeLa cells Balimane et al., 1998; Ganapathy et al., 1998 ; . To characterize the direct interaction between VACV, the L-valyl ester prodrug of ACV, and hPEPT1, the transport of VACV was studied in CHO cells that were transfected with hPEPT1. The uptake of VACV was concentration dependent and saturable Fig. 3 ; . The Km value was 1.64 0.06 mM, which is consistent with the results previously reported in rats Km 1.2 mM ; , rabbits Km 1.3 mM ; , monkey Km 3.4 1.2 mM ; , and Caco-2 cells Km 2.0 mM ; , respectively Smith et al., 1993; Cook et al., 1997; Hu and Sinko, 1997; Sinko and Balimane, 1998 ; . The present results are slightly higher than that reported by others in Caco-2 cells Km 0.3 mM; Lee et al., 1996; de Vrueh et al., 1998 ; and lower than that reported by our group in hPEPT1 XLO Km 5.94; Balimane et al., 1998 ; . Uptake of VACV was dramatically inhibited by known substrates of hPEPT1, including all of the peptides and -lactam antibiotics used in the present study. The Km value from CHO cells with the overexpressed hPEPT1 gene was similar to those obtained from rat and rabbit tissue and Caco-2 cells in vitro. Even though the affinity of VACV was similar in the overexpressed CHO cell system and the in vitro tissue studies, the capacity Jmax ; values could not be directly compared due to the differences in study type. Furthermore, previous results from our laboratory in intact rat intestinal segments suggest that nonmediated diffusion of VACV is minimal Sinko and Balimane, 1998 ; . Therefore, even if there were other transporters involved in the absorption of VACV in vivo, these transporters probably would have an insignificant role. For example, if an organic cation transporter with typical Km values in the micromolar range was involved in VACV uptake, it would be.
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This retrospective study aimed to identify the influence of phosphate-calcium disturbance on the progression of CRF and induction of bone disease and cardiovascular calcification. The study involved 42 patients with CRF in stage 5 regularly followed for 3 to 5 yrs. They were divided into group 1 of 22 pts with normal serum phosphate PO4 ; levels, and group 2 of 20 pts with hyper-PO4 registered in the majority of controls. At the time of the study B mode echocardiogram and native radiograms of the pelvis and the wrist were done. Progression of CRF was faster in group 2 than in group 1 b -0.0577 vs. -0.0288, p 0.003 ; during the study period. Majority of pts was treated with calcium carbonate and 26% of pts from group 1 and 37% of pts from group 2 received vitamine D3. Average BAP 28.8 vs 31 IU and PTH values 580 vs. 339 ng ml ; were similar in both groups. BAP was increased in less than 15% of pts, PTH was high in 69% of pts from group 1 and 58% of pts from group 2 and low in 30% of pts from group 1 and 36% of pts from group 2. Valvular calcifications were found in 4 pts from group 1 and 5 pts from group 2, while arterial calcifications in 1 from group 1 and 2 from group 2. Hyperphosphatemia induced faster CRF progression; valvular and arterial calcifications were rare, while several factors but not only hyper-PO4 contributed to the development of kidney bone disease in predialysis pts. IL9 MICROSURGICAL BREAST RECONSTRUCTION C. Vlastou 1 Department of Plastic, Reconstructive and Aesthetic Surgery, HYGEIA Diagnostic and Therapeutic Center of Athens, Greece. Introduction: Breast reconstruction is an integral part of the surgical management of women with breast cancer, because, by improving the body image, improves patient’ s satisfaction and therefore their quality of life. We are presenting our experience in breast reconstruction with microsurgical free tissue transfers from the lower abdomen. Materials and Methods: During the last five years, 65 patients underwent post-mastectomy breast reconstruction with autologous tissue transfers from the lower abdomen. Microsurgical free tissue transfers were performed in 32 of them, 15 free TRAM flaps and 19 DIEP flaps two bilateral ; . The flap vessels were anastomosed to the internal mammary vessels or to the thoracodorsal vessels at a ratio 3: 1 respectively. Vascular complications occurred in two patients. In one of the two patients circulation was successfully reestablished. Therefore, all free TRAM flaps survived; there was one complete flap loss in the DIEP group. There were no systemic complications and no perioperative deaths. Fat necrosis was observed in about 14% of the patients. In one patient the fat necrosis exceeded 10% of the breast volume and required another surgical procedure for tissue rearrangement, but the final result was not affected. Abdominal donor site morbidity included weakness and bulging of the lower half of the abdominal wall , following free TRAM breast reconstruction in one patient; surgical correction became necessary. Marginal wound necrosis was observed in three patients; it resolved with local wound care. Conclusions: Microsurgical free tissue transfers may provide sufficient autologous breast volume, good contour and adequate projection, with acceptable rates of complications and morbidity IL10 QUALITY OF LIFE IN RHEUMATOID ARTHRITIS A Sarantopoulos 1 Clinical Immunology Unit, 2nd Clinic of Internal Medicine, Aristotle Uiversity of Thessaloniki. Rheumatoid arthritis RA ; is a chronic autoimmune disease.

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COMPETITION LAW AND POLICY IN THE UNITED STATES nine states sought a broad array of remedies in addition to those secured by the United States. The district court rejected virtually all of the non-settling states' proposals, finding that they exceeded the proper scope of the case. Two of the non-settling states have appealed that decision. 35. National Association of Police Equipment Distributors: On July 29, 2002, the DOJ reached a settlement with the National Association of Police Equipment Distributors Inc. NAPED ; that would prohibit NAPED from engaging in an unlawful group boycott of manufacturers that sell police equipment directly to state and local governments under a federal program operated by the General Services Administration GSA ; . NAPED is a trade association of competing distributors and dealers who carry a wide range of police equipment products. 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43products unless authorized by the administration; price and wholesalers and pharmacy margins were fixed by law; the various actors were legally obliged to keep the market supplied; and they could not stop selling a product without administrative authorization ; . The Competition Service thus concluded that there was no effective way for manufacturers to develop a truly independent commercial policy without taking account of competitors or consumers, which is the central factor in determining dominance.138 On appeal, the Spanish Tribunal for the Defense of Competition confirmed the decision.139 The appellant argued that the findings in the Glaxo Dual Pricing140 case concerning the manufacturers' ability to negotiate prices confirmed that Glaxo could behave independently and therefore, be regarded as dominant. The Tribunal responded: "this fact does not mean at all that manufacturers enjoy independence in the fixing of prices, given that Spanish legislation establishes a system of intervention on the price of medicines that need to be authorized by the administration in all phases of commercialization." In other words, the Tribunal does not consider the mere fact that manufacturers negotiate with the authorities on price to mean that they enjoy the sort of independence that is characteristic of a dominant position. Similarly, in Cofares Organon, 141 the Spanish Tribunal for the Defense of Competition stated that, when assessing dominance, regard must be given to the powerful bargaining positions on the demand side. In particular, ".the monopoly position of the national health system must be underlined, due to the fact that its purchases from laboratories, accounting only for the sales through pharmacies, form approximately three quarters of the volume of sales of these laboratories 73.8% in 2000 ; having regard to all the prescribed medicines." The strength of the bargaining power of the Spanish national health system was also referred to in Laboratorios Farmaceuticos, 142 where the Tribunal for the Defense of. 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