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Arizona, California, Colorado, Florida, Idaho, Indiana, Kansas, Louisiana, Michigan, Montana, Nebraska, New Mexico, Ohio, Oklahoma, Oregon, Pennsylvania, Texas, Washington, West Virginia, and Wyoming have adopted a threshold regulatory limit of 5000 ng mL for phenylbutazone, an ARCI class 4 substance. Arkansas and Minnesota have adopted a threshold regulatory limit of 3000 ng mL for phenylbutazone. Delaware, Maryland, New Jersey, and Virginia have adopted a threshold regulatory limit of 2600 ng mL for phenylbutazone. Iowa has adopted a threshold regulatory limit of 2200 ng mL for phenylbutazone. Illinois has adopted a threshold regulatory limit of 2000 ng mL for phenylbutazone. Phenylbutazone is, by rule or law, a race-day medication in Kentucky and New Hampshire. According to the AAEP Guidelines for Drug Detection Times, "a detection time of 48 hours is likely if phenylbutazone has been administered in a multiple dosing regimen and the threshold is 5 g mL. Single intravenous doses of 2 grams of phenylbutazone produce plasma concentrations that are below the 5 g mL threshold by 24 hours after the dose."21 Withdrawal Time Guideline: To our knowledge, no withdrawal time guidelines keyed to a standardized therJournal of Equine Veterinary Science January 2003.
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Various test compounds DMC, L-DMC, DMCM, MEC, AG, SC, and DMBG ; . Apoptogenicity of various carbonyl scavengers decreased in the order of established rate constants of carbonyl trapping Wondrak et al., 2002 ; , suppression of AGE fluorescence formation Ruggiero-Lopez et al., 1999 ; , and inhibition of [14C]lysine-protein cross-linking Lehman and Ortwerth, 2001 ; DMC AG SC DMBG ; , demonstrating a correlation between carbonyl scavenger potency and apoptogenic activity. It is noteworthy that simple derivatization of the prototype agent DMC by esterification, expected to enhance thiol and amino group nucleophilicity and thereby carbonyl scavenger potency Winterbourn and Metodiewa, 1999 ; , potentiated antimelanoma activity of the test compound, suggesting the feasibility of future lead refinement with generation of more potent derivatives active in the micromolar range. Nevertheless, a favorable toxicity profile of DMC, a Food and Drug Administration-approved drug in clinical use as a copper ion chelator for the management of Wilson's disease Cuprimine; Merck, Whitehouse Station, NJ ; , allows the long-term administration of daily dosages of up two 2 g, demonstrating that high tissue concentrations of a prototype carbonyl scavenger can be achieved without inducing systemic adverse effects. Our studies are in agreement with recent research, indicating that RCS are important endogenous small molecule and
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McSwain, J.L., Masaracchia, R.A., Essenberg, R.C., Tucker, J.S., Sauer, J.R. 1992b ; . Amblyomma americanum L. ; : Protein kinase C independent fluid secretion by isolated salivary glands. Experimental Parasitology 74: 324-331. McSwain, J.L., Tucker, J.S., Essenberg, R.C., Sauer, J.R. 1989 ; . Brain factor induced formation of inositol phosphates in tick salivary glands. Insect Biochemistry 19: 343-349. Narumiya, S., Sugimoto, Y., Ushikubi, F. 1999 ; . Prostanoid receptors: Structures, properties and functions. Physiological Reviews 79 4 ; : 1193-1226. Neco, P., Giner, D., Frances, M., Viniegra, S., Gutierrez, L.M. 2003 ; . Differential participation of actinand tubulin-based vesicle transport systems during secretion in bovine chromaffin cells. European Journal of Neuroscience 18: 733-742. Orci, L., Gabbay, K.H., Malaisse, W.J. 1972 ; . Pancreatic beta-cell web: its possible role in insulin secretion. Science 175: 1128-1130. Palmer, M.J., McSwain, J.L., Spatz, M.D., Tucker, J.S., Essenberg, R.C., Sauer, J.R. 1999 ; . Molecular cloning of cAMP-dependent protein kinase catalytic subunits isoforms from the lone star tick, Amblyomma americanum L. ; . Insect Biochemistry and Molecular Biology 29: 43-51. Pedibhotla, V.K., Sarath, G., Sauer, J.R., Stanley-Samuelson, D.W. 1995 ; . Prostaglandin biosynthesis and subcellular localization of prostaglandin H synthase activity in the lone star tick, Amblyomma americanum. Insect Biochemistry and Molecular Biology 25 9 ; : 1027-1039. Qian, Y., Essenberg, R.C., Dillwith, J.W., Bowman, A.S., Sauer, J.R. 1997 ; . A specific prostaglandin E2 receptor and its role in modulating salivary secretion in the female tick, Amblyomma americanum L. ; . Insect Biochemistry and Molecular Biology 27 5 ; : 387-395. Qian, Y., Yuan, J., Essenberg, R.C., Bowman, A.S., Shook, A.L., Dillwith, J.W., Sauer, J.R. 1998 ; . Prostaglandin E2 in the salivary glands of the female tick, Amblyomma americanum L. ; : calcium mobilization and exocytosis. Insect Biochemistry and Molecular Biology 28: 221-228. Risinger, C., Bennett, M.K. 1999 ; . Differential phosphorylation of syntaxin and synaptosome associated protein of 25 kDa SNAP-25 ; isoforms. Journal of Neurochemistry 72 2 ; : 614-624. Roshdy, M.A. 1972 ; . The subgenus Persicargas Ixodoidae, Argasidae, Argas ; . 15. Histology and histochemistry of the salivary glands of A. P. ; persicus Oken ; . Journal of Medical Entomology 9: 143-148. Roshdy, M.A., Coons, L.B. 1975 ; . The subgenus Persicargas Ixodidea: Argasidae: Argas ; . 23. Fine structure of the salivary glands of unfed A. P. ; arboreus Kaiser, Hoogstraal and Kohls ; . Journal of Parasitology 61: 743-752. Sauer, J.R., Essenberg, R.C., Bowman, A.S. 2000 ; . Salivary glands in ixodid ticks: control and mechanism of secretion. Journal of Insect Physiology 46: 1069-1078. Sauer, J.R., Hair, J.A. 1986 ; . Morphology, physiology and behavioral biology of ticks. New York, Chichester, Brisbane, Toronto, Halsted Press: A division of John Wiley & Sons. Schmidt, S.P., Essenberg, R.C., Sauer, J.R. 1981 ; . Evidence for a D1 dopamine receptor in the salivary glands of Amblyomma americanum L. ; . Journal of Cyclic Nucleotide Research 7 6 ; : 375-384. Shipley, M.M., Dillwith, J.W., Bowman, A.S., Essenberg, R.C., Sauer, J.R. 1993 ; . Changes in lipids of the salivary glands of the lone star tick, Amblyomma americanum, during feeding. Journal of Parasitology 79 6 ; : 834-842 and
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