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The purpose of this work is to demonstrate high-throughput automated purification of viral nucleic acids from plasma samples using the QIAGEN BioRobot MDx system, followed by quantitative real-time PCR amplification and detection. Background: There is an increasing demand for automated solutions to purify viral nucleic acids. The QIAGEN BioRobot MDx system offers fully automated purification of viral nucleic acids from a broad range of viruses. The system combines the selective binding properties of a silica-based membrane with a high-throughput automated format. The fully automated process requires a maximum of 2.5 hours to process ninety-six 265 l samples, with no hands-on time. Materials and methods: Negative plasma was spiked with RNA viruses HCV or HIV ; or a DNA virus HBV ; , and viral nucleic acids were purified using the QIAamp Virus BioRobot MDx Kit on the BioRobot MDx workstation. Purified viral RNA or DNA was analyzed using a TaqMan RT-PCR system and in real-time RT-PCR assays using QIAGEN QuantiTect Custom Assays, with primerprobe sets specific for HBV, HCV, and HIV. For each virus, the coefficient of variance for CT values illustrated reproducibility. Conclusions: In this study, we demonstrated that the QIAGEN BioRobot MDx system enables fully automated purification of high-quality viral RNA and DNA purification from plasma samples without detectable cross-contamination. Purified RNA and DNA was highly suitable for use in real-time PCR and gave reproducible CT values. These results demonstrate the capability for automated, high-throughput purification of viral nucleic acids using QIAGEN technology platforms and testosterone!


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